{"id":3051,"date":"2020-12-17T10:23:10","date_gmt":"2020-12-17T09:23:10","guid":{"rendered":"http:\/\/www.e-rihs.it\/?page_id=3051"},"modified":"2024-05-07T11:19:37","modified_gmt":"2024-05-07T10:19:37","slug":"bioluminescence","status":"publish","type":"page","link":"https:\/\/www.e-rihs.it\/en\/bioluminescence\/","title":{"rendered":"Bioluminescence"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.7.7″ _module_preset=”default”][et_pb_row column_structure=”2_5,3_5″ _builder_version=”4.7.7″ _module_preset=”default” hover_enabled=”0″ sticky_enabled=”0″][et_pb_column type=”2_5″ _builder_version=”4.7.7″ _module_preset=”default”][et_pb_image src=”http:\/\/www.e-rihs.it\/wp-content\/uploads\/2022\/03\/Bioluminesceza.jpg” title_text=”Bioluminescenza” force_fullwidth=”on” _builder_version=”4.7.7″ _module_preset=”default”][\/et_pb_image][\/et_pb_column][et_pb_column type=”3_5″ _builder_version=”4.7.7″ _module_preset=”default”][et_pb_text _builder_version=”4.7.7″ _module_preset=”default”]<\/p>\n

LABORATORIO: CNR ISPC<\/strong><\/p>\n

 <\/p>\n

NOME STRUMENTO<\/strong><\/p>\n

Bioluminometro 3M\u2122 Clean-Trace\u2122 NG Luminometer, Swabs 3M Clean-Trace Surface3M\u2122, Clean-Trace\u2122 Water<\/p>\n

 <\/p>\n

INFORMAZIONI GENERALI<\/strong><\/p>\n

II bioluminometro \u00e8 uno strumento che permette di verificare istantaneamente la contaminazione microbica di una superficie o di un liquido, grazie a una reazione di bioluminescenza.<\/p>\n

II bioluminometro “Iegge” un segnale luminoso che viene emesso da un tampone che \u00e8 stato passato sulla superficie contaminata o nell\u2019acqua da analizzare. Durante il campionamento, il tampone raccoglie una molecola cellulare, I’ATP (adenosina trifosfato) presente in tutti gli organismi vivi. Quando I’ATP viene posta in contatto con il mix di reazione presente nel tampone (contenente luciferina e luciferasi), si svolge una reazione che ha come effetto anche la produzione di luce (fotoni). Questa luce viene misurata dal bioluminometro e convertita in un valore numerico riportato sui display. Pi\u00f9 elevata \u00e8 la contaminazione microbica, pi\u00f9 sar\u00e0 elevata la quantit\u00e0 di ATP raccolta e, di conseguenza, la luce prodotta e il valore misurato dal bioluminometro.<\/p>\n

 <\/p>\n

DETTAGLI TECNICI<\/strong><\/p>\n

ATP (adenosina trifosfato) \u00e8 una molecola presente in tutte le cellule viventi, e d\u00e0 una misura diretta della concentrazione microbica e della salute dei microorganismi. L\u2019ATP viene quantificato misurando la luce prodotta attraverso la reazione fra l\u2019ATP e l\u2019enzima luciferasi (naturalmente presente nelle lucciole) usando un luminometro. La quantit\u00e0 di luce prodotta \u00e8 direttamente proporzionale alla quantit\u00e0 di organismi viventi presenti nel campione.<\/p>\n

Applicazione in-situ per il rilevamento dell\u2019attivit\u00e0 microbica su tutti i tipi di materiali del patrimonio culturale. Nei programmi di dosaggio dei biocidi che interessano il controllo dei biodeteriogeni (verificare l’efficienza del biocida\/disinfettante nel tempo, stabilire la sua minima concentrazione inibente).<\/p>\n

 <\/p>\n

Referente:
Oana A. Cuzman oanaadriana.cuzman@cnr.it<\/a><\/p>\n

 <\/p>\n

Scarica la scheda completa<\/a><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

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